Dr. Caleb R Stoltzfus

Dr. Caleb R Stoltzfus

Director of Research and Development at Alpenglow Biosciences

Posts by Collection

news

CytoMAP Version 1.4.0

Published:

CytoMAP version 1.4.0 is up and running! It barely even looks like the same program as version 1.0. Check it out if you want to do single cell spatial analysis.

publications

The Two-Photon Bazooka: A New Way of Optically Screening Randomly Mutagenized Libraries of Fluorescent Proteins

Published in Biophysical Journal, 2014

Abstract

Recommended citation: Barnett, Lauren M., Caleb Stoltzfus, Geoffrey Wicks, Mikhail Drobizhev, Alexandr Mikhailov, Aleksander Rebane, and Thomas E. Hughes. "The two-photon bazooka: A new way of optically screening randomly mutagenized libraries of fluorescent proteins." Biophysical Journal 106, no. 2 (2014): 810a-811a. https://doi.org/10.1016/j.bpj.2013.11.4445

A Multidimensional Screening Method for the Selection of Two-Photon Enhanced Fluorescent Proteins

Published in International Society for Optics and Photonics, 2014

Abstract

Recommended citation: Stoltzfus, Caleb, Lauren Barnett, Aleksander Rebane, Thomas Hughes, Mikhail Drobizhev, Geoffrey Wicks, and Alexandr Mikhailov. "A multidimensional screening method for the selection of two-photon enhanced fluorescent proteins." In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VI, vol. 8956, p. 895611. International Society for Optics and Photonics, 2014. https://doi.org/10.1117/12.2039873

Multiphoton Photochemistry of Red Fluorescent Proteins in Solution and Live Cells

Published in The Journal of Physical Chemistry B, 2014

Abstract

Recommended citation: Drobizhev, Mikhail, Caleb Stoltzfus, Igor Topol, Jack Collins, Geoffrey Wicks, Alexander Mikhaylov, Lauren Barnett, Thomas E. Hughes, and Aleksander Rebane. "Multiphoton photochemistry of red fluorescent proteins in solution and live cells." The Journal of Physical Chemistry B 118, no. 31 (2014): 9167-9179. https://doi.org/10.1021/jp502477c

Optimizing Ultrafast Wide Field-of-View Illumination for High-Throughput Multi-Photon Imaging and Screening of Mutant Fluorescent Proteins

Published in International Society for Optics and Photonics, 2017

Abstract

Recommended citation: Stoltzfus, Caleb, Alexandr Mikhailov, and Aleksander Rebane. "Optimizing ultrafast wide field-of-view illumination for high-throughput multi-photon imaging and screening of mutant fluorescent proteins." In Multiphoton Microscopy in the Biomedical Sciences XVII, vol. 10069, p. 1006924. International Society for Optics and Photonics, 2017. https://doi.org/10.1117/12.2250048

Ultra-low Dose Aerosol Infection of Mice with Mycobacterium tuberculosis More Closely Models Human Tuberculosis

Published in Cell Host & Microbe, 2021

Abstract

Recommended citation: Plumlee, Courtney R., Duffy, Fergal J., Gern, Benjamin H., Delahaye, Jared L., Cohen, Sara B., Stoltzfus, Caleb R., Rustad, Tige R. et al. "Ultra-low Dose Aerosol Infection of Mice with Mycobacterium tuberculosis More Closely Models Human Tuberculosis" Cell Host & Microbe 29, no. 1 (2021): 68-82. https://doi.org/10.1016/j.chom.2020.10.003

Innate cell microenvironments in lymph nodes shape the generation of T cell responses during type I inflammation

Published in Science Immunology, 2021

Abstract

Recommended citation: Leal, Joseph M., Huang, Jessica Y., Kohli, Karan, Stoltzfus, Caleb R., Lyons-Cohen, Miranda R., Olin, Brandy E., Gale Jr., Michael, Gerner, Michael Y. "Innate cell microenvironments in lymph nodes shape the generation of T cell responses during type I inflammation" Science Immunology 6, no. 56 (2021): eabb9435 https://doi.org/10.1126/sciimmunol.abb9435

Multi-Parameter Quantitative Imaging of Tumor Microenvironments Reveals Perivascular Immune Niches Associated with Anti-Tumor Immunity

Published in BioRxiv, 2021

Abstract

Recommended citation: Caleb R Stoltzfus, Ramya Sivakumar, Leo Kunz, Brandy E Olin Pope, Elena Menietti, Dario Speziale, Roberto Adelfio, Marina Bacac, Sara Colombetti, Mario Perro, Michael Y Gerner "Multi-Parameter Quantitative Imaging of Tumor Microenvironments Reveals Perivascular Immune Niches Associated with Anti-Tumor Immunity" bioRxiv 2021.06.17.448881; doi: https://doi.org/10.1101/2021.06.17.448881 https://doi.org/10.1101/2021.06.17.448881

A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues

Published in Nature Methods, 2022

Abstract

Recommended citation: Glaser, Adam K., Bishop, Kevin W., Barner, Lindsey A., Susaki, Etsuo A., Kubota, Shimpei I., Gao, Gan, Serafin, Robert B., Balaram, Pooja, Turschak, Emily, Nicovich, Philip R., Lai, Hoyin, Lucas, Luciano A. G., Yi, Yating, Nichols, Eva K., Huang, Hongyi, Reder, Nicholas P., Wilson, Jasmine J., Sivakumar, Ramya, Shamskhou, Elya, Stoltzfus, Caleb R., Wei, Xing, Hempton, Andrew K., Pende, Marko, Murawala, Prayag, Dodt, Hans-Ulrich, Imaizumi, Takato, Shendure, Jay, Beliveau, Brian J., Gerner, Michael Y., Xin, Li, Zhao, Hu, True, Lawrence D., Reid, Clay R., Chandrashekar, Jayaram, Ueda, Hiroki R., Svoboda, Karel, Lui, Jonathan T.C. "A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues." Nature Methods 19, no. 5 (2022): 613-619. https://doi.org/10.1038/s41592-022-01468-5

research

talks

teaching

Holography Lab (TA)

Undergraduate course, Montana State University, Department of Physics, 2010

Under the supervision of Professor Randy Babbitt I taught lab sections demonstrating how to make holograms. I occasionally also taught lectures.

Advanced Optics (TA)

Undergraduate course, Montana State University, Department of Physics, 2016

Under the supervision of Professor Rufus Cone I graded homework and held office hours. This course covered new developments in optics laser physics. Provided a good foundation in wave optics, nonlinear optics, integrated optics, and spectroscopy.

Introductory Physics (TA)

Undergraduate course, Montana State University, Department of Physics, 2016

Under the supervision of Profesor Gregory Francis I taught a lab section, graded homework, and graded exams. This introductory physics course covered simple harmonic motion; electric forces and fields; dc electric circuits; magnetic forces and fields; and magnetic induction and motors.