Multi-Immersion Open-Top Light-Sheet Microscope for High-Throughput Imaging of Cleared Tissues

Published in Nature Communications, 2019

Recommended citation: Glaser, Adam K., Reder, Nicholas P., Chen, Ye, Yin, Chengbo, Wei, Linpeng, Kang, Soyoung, Barner, Lindsey A. et al. "Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues." Nature Communications 10, no. 1 (2019): 1-8.


Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of clearing protocols, which will facilitate wide adoption by preclinical researchers and clinical laboratories. In particular, the open-top geometry provides unsurpassed versatility to interface with a wide range of accessory technologies in the future.

Download paper here